Collagen Type I antibody

Catalog No. ABIN372600

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Mouse, Mammalian, Fish, Sheep, Amphibian, Avian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Specific for the ~140 kDa telopeptide portion of the Collagen I Alpha-1 polypeptide.

Cross-Reactivity (Details): Species reactivity (expected):all Mammals, Birds and Amphibians.
Species reactivity (tested):Human, Mouse, Rat and Sheep.

Purification: Affinity Chromatography.

Immunogen: Peptide from the Human Collagen I Alpha-1 telopeptide sequence.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemitry: 1/100. The antibody works well for Immunohistochemistry on Paraformaldehyde-Fixed Sectionswith a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in 10 mMsodium citrate ( pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections ofFormaldehyde-Fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen Ithat has formed fibrils in the extracellular matrix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452