Tryptophan Hydroxylase 2 antibody (pSer19)

Catalog No. ABIN372731

The Rabbit Polyclonal anti-Tryptophan Hydroxylase 2 antibody is suitable to detect Tryptophan Hydroxylase 2 in samples from Mouse, Rat, Cow and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Tryptophan Hydroxylase 2 antibody (pSer19) (ABIN372731)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reactivity: Mouse, Rat, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tryptophan Hydroxylase 2 Antibody

Binding Specificity: pSer19

Specificity: Specific for the ~55k Tryptophan Hydroxylase protein phosphorylated at Ser19.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Mouse and Zebrafish.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat Tryptophan Hydroxylase 2 (TPH2).

Isotype: IgG

Application Details

Application Notes: Western blotting: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Alternative Name: Tryptophan 5-Hydroxylase 2 (TPH2)

Background: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).Synonyms: NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

Gene ID: 317675

NCBI Accession: NP_776211

UniProt: Q8CGU9