OGG1 antibody (Internal Region)

Catalog No. ABIN372767

The Goat Polyclonal anti-OGG1 antibody is suitable to detect OGG1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for OGG1 antibody (Internal Region) (ABIN372767)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This OGG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-OGG1 Antibody

Binding Specificity: Internal Region

Specificity: Recognizes 8-oxoguanine DNA Glycosylase Homolog 1 (OGG1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human, Rat.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-HPKTSQAKGPSPLANK) from an Internal region of Mouse OGG1.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/28000. Western Blot: 0.1-0.3 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris Saline Buffer, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: OGG1

Background: The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at 8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6 diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. N glycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion. Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N terminal region in common. Many alternative splice variants for this gene have been described, but the full length nature for every variant has not been determined. The N terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.Synonyms: MMH, MUTM, N-glycosylase/DNA lyase, OGH1

Gene ID: 81528

NCBI Accession: NP_110497

UniProt: O70249

Pathways: DNA Damage Repair