Androgen Receptor antibody (N-Term)

Catalog No. ABIN374452

This Goat Polyclonal antibody specifically detects Androgen Receptor in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Pig, Dog and Cow.

Quick Overview for Androgen Receptor antibody (N-Term) (ABIN374452)

Target: Androgen Receptor (AR)

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Androgen Receptor Antibody

Binding Specificity: N-Term

Sequence: EVQLGLGRVY PRPPSC

Specificity: This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_000035

Application Details

Application Notes: Peptide ELISA: 1/64,000. Western Blot: 0.3-2.0 μg/mL. Detects a band of Approx 95 kDa in Human Brain and HumanHeart lysates(Predicted Molecular Weight: 95 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor

Background: The androgen receptor (AR) is a 110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis.Synonyms: DHTR, Dihydrotestosterone receptor, NR3C4, Nuclear receptor subfamily 3 group C member 4

Gene ID: 367, 9606

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding