PEPCK antibody (Internal Region)

Catalog No. ABIN374610

Cited in 2+ publications. The Goat Polyclonal anti-PEPCK antibody (ABIN374610) specifically detects PEPCK in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Cow, Dog and Pig samples.

Quick Overview for PEPCK antibody (Internal Region) (ABIN374610)

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PEPCK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PEPCK Antibody

Binding Specificity: Internal Region

Sequence: KPWKPGDKEP CAH

Specificity: This antibody recognizes PCK2. This antibody is expected to recognise isoform 1 only, no crossreactivity with PCK1.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse, Rat, Guinea Pig.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004554.2. Genename: PCK2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Liver lysates and inlysates of cell line HepG2 (calculated MW of 70.0 kDa according to NP_004554.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-PEPCK antibody (ABIN374610)

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Idnurm: "Light sensing in Aspergillus fumigatus highlights the case for establishing new models for fungal photobiology." in: mBio, Vol. 4, Issue 3, pp. e00260-13, (2013) (PubMed).

Target Details for PEPCK

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Alternative Name: PCK2

Background: PCK2 is a a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.Synonyms: EC=4.1.1.32, PCK-2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase

Gene ID: 5106

NCBI Accession: NP_001018083

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis