Glutathione Peroxidase 1 antibody (Internal Region)

Catalog No. ABIN374697

The Goat Polyclonal anti-Glutathione Peroxidase 1 antibody (ABIN374697) specifically detects Glutathione Peroxidase 1 in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for Glutathione Peroxidase 1 antibody (Internal Region) (ABIN374697)

Target: Glutathione Peroxidase 1 (GPX1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Glutathione Peroxidase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Glutathione Peroxidase 1 Antibody

Binding Specificity: Internal Region

Sequence: C-REALPAPSDD ATA

Specificity: This antibody is expected to recognise Human Glutathione Peroxidase 1 Isoform 1 only (NP_000572.2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Syntehtic Peptide from the internal region of Human GPX1 (NP_000572.2). Genename: GPX1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Western blot: 1-3 μg/mL. Approx 22 kDa band observed in Human Liver and Kidney lysates(calculated MW of 22.2 kDa according to NP_000572.2). Immunohistochemistry: 3.5 μg/mL. In paraffin embedded Human Cerebral Cortex shows staining of microglia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Alternative Name: Glutathione Peroxidase 1 / GPX1

Background: Glutathione peroxidases(GPxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. This protein is one of the most important antioxidant enzymes in humans. This protein has been reported to protect cultured breast cancer cells from CD95-induced apoptosis and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. There are several isoforms which differ in their primary structure and localization. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site. One allele of the Glutathione Peroxidase 1 gene is significantly associated with an increased breast cancer risk.Synonyms: Cellular glutathione peroxidase, GSHPx-1

Gene ID: 2876

NCBI Accession: NP_000572

UniProt: P07203

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome