Liver Arginase antibody (Arg1, C-Term)

Catalog No. ABIN374811

The Goat Polyclonal anti-Liver Arginase antibody (ABIN374811) specifically detects Liver Arginase in WB. The antibody is reactive with Human and Dog samples.

Quick Overview for Liver Arginase antibody (Arg1, C-Term) (ABIN374811)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Liver Arginase Antibody

Binding Specificity: Arg1, C-Term

Sequence: C-REGNHKPIDY LNPPK

Specificity: This antibody detects Arginase-1.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_000036.2 Genename: ARG1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 2000. Western blot: 0.03-0.1 μg/mL. Approx 35 kDa band observed in Human Liver lysates(calculated MW of 34.5 kDa according to NP_000036.2. Additional bands of unknown identity were also consistently observed at 19-25 kDa. Thiesebands were successfully blocked by incubation with the immunising peptide. We wouldappreciate any feedback from people in the field - have any such results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase-1

Background: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.Synonyms: ARG1, Liver-type arginase, Type I arginase

Gene ID: 383, 9606

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin