ITGA4 antibody (PE)

Catalog No. ABIN375534

This anti-ITGA4 antibody is a Rat Monoclonal antibody detecting ITGA4 in FACS. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for ITGA4 antibody (PE) (ABIN375534)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: PS-2

Product Details anti-ITGA4 Antibody (PE)

Specificity: Mouse/Human CD49d, Mr 150 kDa

Characteristics: Rat Anti-Mouse CD49d-PE

Purification: Purified

Immunogen: P815 cells

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Adhesion - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 2 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-ITGA4 antibody (PE) (ABIN375534)

Fistonich, Zehentmeier, Bednarski, Miao, Schjerven, Sleckman, Pereira: "Cell circuits between B cell progenitors and IL-7+ mesenchymal progenitor cells control B cell development." in: The Journal of experimental medicine, Vol. 215, Issue 10, pp. 2586-2599, (2018) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: VLA-4 belongs to the β 1 -integrin family, which functions both as cell-matrix and cell-cell receptors. As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive.

Pathways: Integrin Complex