TNF alpha antibody

Catalog No. ABIN375619

This Rat Monoclonal antibody specifically detects TNF alpha in ELISA, FACS and D. It exhibits reactivity toward Mouse.

Quick Overview for TNF alpha antibody (ABIN375619)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Detection (D)

Clone: MP6-XT22

Product Details anti-TNF alpha Antibody

Specificity: Mouse/Chicken TNF-α

Characteristics: Rat Anti-Mouse TNF-a-UNLB

Purification: Purified

Immunogen: E. coli-expressed mouse TNF-alpha

Isotype: IgG1

Application Details

Application Notes:

• Applications: ELISA-Capture - Quality tested , ELISA-Detection - Quality tested , FC - Quality tested , ELISPOT-Capture - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Neut - Reported in literature , Multiplex-Detection - Reported in literature
• Working Dilutions: ELISA Purified (UNLB) antibody ≤ 2 g/mL BIOT conjugate 1:2,000 - 1:4,000 Flow Cytometry FITC conjugate ≤ 1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-a

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome