IFITM3 antibody (N-Term)

Catalog No. ABIN387989

The Rabbit Polyclonal anti-IFITM3 antibody has been validated for WB, IF and IHC (p). It is suitable to detect IFITM3 in samples from Human and Mouse. There are 6+ publications available.

Quick Overview for IFITM3 antibody (N-Term) (ABIN387989)

Target: IFITM3 (Interferon-Induced Transmembrane Protein 3 (IFITM3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFITM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB13975

Product Details anti-IFITM3 Antibody

Binding Specificity: AA 1-30, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Interferon-inducible protein (IFITM3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Interferon-inducible protein (IFITM3).

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100~500. WB: 1:1000. WB: 1:2000. WB: 1:1000. IHC-P-Leica: 1:100. IHC-P-Leica: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-IFITM3 antibody (ABIN387989)

Savidis, Perreira, Portmann, Meraner, Guo, Green, Brass: "The IFITMs Inhibit Zika Virus Replication." in: Cell reports, Vol. 15, Issue 11, pp. 2323-30, (2017) (PubMed).

Mäkelä, Österlund, Westenius, Latvala, Diamond, Gale, Julkunen: "RIG-I Signaling Is Essential for Influenza B Virus-Induced Rapid Interferon Gene Expression." in: Journal of virology, Vol. 89, Issue 23, pp. 12014-25, (2015) (PubMed).

John, Chin, Perreira, Feeley, Aker, Savidis, Smith, Elia, Everitt, Vora, Pertel, Elledge, Kellam, Brass: "The CD225 domain of IFITM3 is required for both IFITM protein association and inhibition of influenza A virus and dengue virus replication." in: Journal of virology, Vol. 87, Issue 14, pp. 7837-52, (2013) (PubMed).

Everitt, Clare, Pertel, John, Wash, Smith, Chin, Feeley, Sims, Adams, Wise, Kane, Goulding, Digard, Anttila, Baillie, Walsh, Hume, Palotie, Xue, Colonna, Tyler-Smith, Dunning, Gordon, Smyth, Openshaw et al.: "IFITM3 restricts the morbidity and mortality associated with influenza. ..." in: Nature, Vol. 484, Issue 7395, pp. 519-23, (2012) (PubMed).

Feeley, Sims, John, Chin, Pertel, Chen, Gaiha, Ryan, Donis, Elledge, Brass: "IFITM3 inhibits influenza A virus infection by preventing cytosolic entry." in: PLoS pathogens, Vol. 7, Issue 10, pp. e1002337, (2011) (PubMed).

Brass, Huang, Benita, John, Krishnan, Feeley, Ryan, Weyer, van der Weyden, Fikrig, Adams, Xavier, Farzan, Elledge: "The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus." in: Cell, Vol. 139, Issue 7, pp. 1243-54, (2010) (PubMed).

Target Details for IFITM3

Target: IFITM3 (Interferon-Induced Transmembrane Protein 3 (IFITM3))

Alternative Name: Interferon-inducible protein (IFITM3)

Background: The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.

Molecular Weight: 14632

Gene ID: 10410

NCBI Accession: NP_066362

UniProt: Q01628