PDX1 antibody (C-Term)

Catalog No. ABIN388773

The Rabbit Polyclonal anti-PDX1 antibody (Clone RB02861-02862) (ABIN388773) specifically detects PDX1 in WB. The antibody is reactive with Human samples.

Quick Overview for PDX1 antibody (C-Term) (ABIN388773)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB02861-02862

Product Details anti-PDX1 Antibody

Binding Specificity: AA 429-459, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-459 amino acids from the C-terminal region of human PDX1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: PDX1

Background: PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.

Molecular Weight: 54122

Gene ID: 8050

NCBI Accession: NP_001128496, NP_001159630, NP_003468

UniProt: O00330

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location