USP4 antibody (C-Term)

Catalog No. ABIN2839175

The Rabbit Polyclonal anti-USP4 antibody has been validated for WB and IHC (p). It is suitable to detect USP4 in samples from Human. There is 1 publication available.

Quick Overview for USP4 antibody (C-Term) (ABIN2839175)

Target: USP4 (Ubiquitin Specific Peptidase 4 (USP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4269

Product Details anti-USP4 Antibody

Binding Specificity: AA 903-933, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This USP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 903-933 amino acids from the C-terminal region of human USP4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-USP4 antibody (ABIN2839175)

Laurell, Velázquez-Fernández, Lindsten, Juhlin, Enberg, Geli, Höög, Kjellman, Lundeberg, Hamberger, Larsson, Nilsson, Bäckdahl: "Transcriptional profiling enables molecular classification of adrenocortical tumours." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 161, Issue 1, pp. 141-52, (2009) (PubMed).

Target Details for USP4

Target: USP4 (Ubiquitin Specific Peptidase 4 (USP4))

Alternative Name: USP4

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Molecular Weight: 108565

Gene ID: 7375

NCBI Accession: NP_001238806, NP_003354, NP_955475

UniProt: Q13107