ARFGAP1 antibody (C-Term)

Catalog No. ABIN388996

This Rabbit Polyclonal antibody specifically detects ARFGAP1 in WB and IHC (p). It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for ARFGAP1 antibody (C-Term) (ABIN388996)

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4855

Product Details anti-ARFGAP1 Antibody

Binding Specificity: AA 377-406, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the C-terminal region of human ARFGAP1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:8000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ARFGAP1 antibody (ABIN388996)

Nishimura, Uchida, Yachi, Kudlyk, Lupashin, Inoue, Taguchi, Arai: "Oxysterol-binding protein (OSBP) is required for the perinuclear localization of intra-Golgi v-SNAREs." in: Molecular biology of the cell, Vol. 24, Issue 22, pp. 3534-44, (2013) (PubMed).

Zhang, Major, Takanashi, Camp, Nishiya, Peters, Ginsberg, Jian, Randazzo, Schultz, Moon, Ding: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, (2007) (PubMed).

Target Details for ARFGAP1

Target: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Alternative Name: ARFGAP1

Background: ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.

Molecular Weight: 44668

Gene ID: 55738

NCBI Accession: NP_001268411, NP_001268412, NP_001268413, NP_060679, NP_783202

UniProt: Q8N6T3

Pathways: p53 Signaling, ER-Nucleus Signaling, Unfolded Protein Response