Retinoblastoma 1 antibody (pSer249)

Catalog No. ABIN389641

This Rabbit Polyclonal antibody specifically detects Retinoblastoma 1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Retinoblastoma 1 antibody (pSer249) (ABIN389641)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB17434

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: pSer249

Specificity: This Phospho-Rb-S249 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S249 of human Rb.

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Rb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S249 of human Rb.

Isotype: Ig Fraction

Application Details

Application Notes: WB = 1:1000, IHC (p) = 1:50-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.41 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: RB1 likely acts as a regulator of other genes. It forms a complex with adenovirus E1A and with SV40 large T antigen, acts as a tumor suppressor, and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. RB1 is a potent inhibitor of E2F-mediated trans-activation, and also recruits and targets histone methyltransferase SUV39H1 leading to epigenetic transcriptional repression. This protein inhibits the intrinsic kinase activity of TAF1. Defects in RB1 are the cause of childhood cancer retinoblastoma (RB), a congenital malignant tumor that arises from the nuclear layers of the retina. Defects in RB1 are also a cause of bladder cancer and osteogenic sarcoma.
Synonyms: Retinoblastoma-associated protein, PP110, P105-RB, RB, RB1,

Molecular Weight: 106159 DA

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy