MTOR antibody (AA 2459-2488)

Catalog No. ABIN390217

This Rabbit Polyclonal antibody specifically detects MTOR in WB, IF and IHC (p). It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for MTOR antibody (AA 2459-2488) (ABIN390217)

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB13347

Product Details anti-MTOR Antibody

Binding Specificity: AA 2459-2488

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This mTOR (FRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2459-2488 amino acids from human mTOR (FRAP1).

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MTOR antibody (ABIN390217)

Yang, Cheng, Zhou, Zhu, Hu, Yang: "Overexpression of X-Box Binding Protein 1 (XBP1) Correlates to Poor Prognosis and Up-Regulation of PI3K/mTOR in Human Osteosarcoma." in: International journal of molecular sciences, Vol. 16, Issue 12, pp. 28635-46, (2016) (PubMed).

Liu, Wu, Huang: "Orientin protects myocardial cells against hypoxia-reoxygenation injury through induction of autophagy." in: European journal of pharmacology, Vol. 776, pp. 90-8, (2016) (PubMed).

Cao, Dong, Meng, Liu, Liao, Liu: "MiR-511 inhibits growth and metastasis of human hepatocellular carcinoma cells by targeting PIK3R3." in: Tumour biology, Vol. 36, Issue 6, pp. 4453-9, (2015) (PubMed).

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: mTOR (FRAP1)

Background: FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

Molecular Weight: 288892

Gene ID: 2475

NCBI Accession: NP_004949

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect