DCLK1 antibody (AA 690-720)

Catalog No. ABIN391324

Product Details anti-DCLK1 Antibody

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Binding Specificity: AA 690-720

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids of human DCAMKL1.

Clone: RB3161

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:1000. WB: 1:8000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-DCLK1 antibody (ABIN391324)

Kawamura, Takemoto, Nishimoto, Ueno, Hosoyama, Shirasawa, Tanaka, Kugimiya, Harada, Hamano: "Enhancement of cytotoxic effects of gemcitabine by Dclk1 inhibition through suppression of Chk1 phosphorylation in human pancreatic cancer cells." in: Oncology reports, Vol. 38, Issue 5, pp. 3238-3244, (2018) (PubMed).

Ushiama, Ishimaru, Narukawa, Yoshioka, Kozuka, Watanabe, Tsunoda, Osakabe, Asakura, Masuzaki, Abe: "Catecholamines Facilitate Fuel Expenditure and Protect Against Obesity via a Novel Network of the Gut-Brain Axis in Transcription Factor Skn-1-deficient Mice." in: EBioMedicine, Vol. 8, pp. 60-71, (2017) (PubMed).

Gao, Wang, Xu, Wen, Liu, An: "DCLK1 is up-regulated and associated with metastasis and prognosis in colorectal cancer." in: Journal of cancer research and clinical oncology, Vol. 142, Issue 10, pp. 2131-40, (2017) (PubMed).

Leppänen, Helminen, Huhta, Kauppila, Miinalainen, Ronkainen, Saarnio, Lehenkari, Karttunen: "Doublecortin-like kinase 1-positive enterocyte - a new cell type in human intestine." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 124, Issue 11, pp. 958-965, (2017) (PubMed).

Schellnegger, Quante, Rospleszcz, Schernhammer, Höhl, Tobiasch, Pastula, Brandtner, Abrams, Strauch, Schmid, Vieth, Wang, Quante: "Goblet Cell Ratio in Combination with Differentiation and Stem Cell Markers in Barrett Esophagus Allow Distinction of Patients with and without Esophageal Adenocarcinoma." in: Cancer prevention research (Philadelphia, Pa.), Vol. 10, Issue 1, pp. 55-66, (2017) (PubMed).

Nakata, Shimizu, Nagata, Ito, Fujii, Suzuki, Kawamoto, Ishibashi, Kuno, Anzai, Murano, Mizutani, Oshima, Tsuchiya, Nakamura, Hozumi, Watanabe, Okamoto: "Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine." in: Data in brief, Vol. 10, pp. 551-556, (2017) (PubMed).

Gross, Balderes, Liu, Asfaha, Gu, Wang, Sussel: "Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential." in: American journal of physiology. Gastrointestinal and liver physiology, pp. ajpgi.00244.2015, (2015) (PubMed).

Shimizu, Okamoto, Ito, Fujii, Nakata, Suzuki, Murano, Mizutani, Tsuchiya, Nakamura, Hozumi, Watanabe: "Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine." in: PeerJ, Vol. 2, pp. e370, (2014) (PubMed).

Gonzalez, Williamson, Piedrahita, Blikslager, Magness: "Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration." in: PLoS ONE, Vol. 8, Issue 6, pp. e66465, (2013) (PubMed).

Trivedi, Wiber, El-Zimaity, Brubaker: "Glucagon-like peptide-2 increases dysplasia in rodent models of colon cancer." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 8, pp. G840-9, (2012) (PubMed).

Yu, Chen, Zhang, Li, Xu, Wang, Ai, Liu: "Krüppel-like factor 4 regulates intestinal epithelial cell morphology and polarity." in: PLoS ONE, Vol. 7, Issue 2, pp. e32492, (2012) (PubMed).

Gerbe, van Es, Makrini, Brulin, Mellitzer, Robine, Romagnolo, Shroyer, Bourgaux, Pignodel, Clevers, Jay: "Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium." in: The Journal of cell biology, Vol. 192, Issue 5, pp. 767-80, (2011) (PubMed).

Gerbe, Brulin, Makrini, Legraverend, Jay: "DCAMKL-1 expression identifies Tuft cells rather than stem cells in the adult mouse intestinal epithelium." in: Gastroenterology, Vol. 137, Issue 6, pp. 2179-80; author reply 2180-1, (2009) (PubMed).

Dekaney, Gulati, Garrison, Helmrath, Henning: "Regeneration of intestinal stem/progenitor cells following doxorubicin treatment of mice." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 297, Issue 3, pp. G461-70, (2009) (PubMed).

May, Riehl, Hunt, Sureban, Anant, Houchen et al.: "Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple ..." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 3, pp. 630-7, (2008) (PubMed).

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCAMKL1 (DCLK1 Products)

Synonyms: DCLK1 antibody, DCAMKL1 antibody, dcamkl1 antibody, dclk1 antibody, wu:fc51f11 antibody, zgc:153709 antibody, MGC145348 antibody, CL1 antibody, CLICK1 antibody, DCDC3A antibody, DCLK antibody, 1700113D08Rik antibody, 2810480F11Rik antibody, AI836758 antibody, Click-I antibody, Cpg16 antibody, Dcamkl1 antibody, Dcl antibody, Dclk antibody, mKIAA0369 antibody, Ania4 antibody, doublecortin like kinase 1 antibody, doublecortin-like kinase 1a antibody, serine/threonine-protein kinase DCLK1 antibody, doublecortin-like kinase 2 antibody, doublecortin-like kinase 1 antibody, DCLK1 antibody, dclk1a antibody, LOC587664 antibody, dclk2 antibody, LOC100539645 antibody, Dclk1 antibody

Background: Doublecortin-like kinase (DCAMKL1)(Ser/Thr protein kinase family) is essential for proper neurogenesis, neuronal migration, and axonal wiring. DCAMKL1 is involved in a calcium-signaling pathway controling neuronal migration in the developing brain, and participates in functions of the mature nervous system. DCAMKL1 protein shares high homology with doublecortin (DCX). DCLK, but not DCX, is highly expressed in regions of active neurogenesis in the neocortex and cerebellum. DCAMKL1 controls mitotic division by regulating spindle formation and also determines the fate of neural progenitors during cortical neurogenesis.

Molecular Weight: 82224

Gene ID: 9201

NCBI Accession: NP_001182344, NP_001182345, NP_004725

UniProt: O15075