Dihydrofolate Reductase antibody (C-Term)

Catalog No. ABIN391566

The Rabbit Polyclonal anti-Dihydrofolate Reductase antibody (Clone RB19204) (ABIN391566) specifically detects Dihydrofolate Reductase in WB and IHC (p). The antibody is reactive with Mouse and Rat samples.

Quick Overview for Dihydrofolate Reductase antibody (C-Term) (ABIN391566)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB19204

Product Details anti-Dihydrofolate Reductase Antibody

Binding Specificity: AA 135-164, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of mouse DHFR.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background: Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Molecular Weight: 21606

Gene ID: 13361

NCBI Accession: NP_034179

UniProt: P00375

Pathways: Mitotic G1-G1/S Phases