PLK1 antibody (C-Term)

Catalog No. ABIN392446

This Rabbit Polyclonal antibody specifically detects PLK1 in WB and IHC (p). It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for PLK1 antibody (C-Term) (ABIN392446)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB00878

Product Details anti-PLK1 Antibody

Binding Specificity: AA 573-603, C-Term

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573-603 amino acids from the C-terminal region of human PLK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PLK1 antibody (ABIN392446)

Negishi, Kumano, Nishida: "Polo-like kinase 1 is required for localization of Posterior End Mark protein to the centrosome-attracting body and unequal cleavages in ascidian embryos." in: Development, growth & differentiation, Vol. 53, Issue 1, pp. 76-87, (2011) (PubMed).

Li, Zhou, Ye, Cheng, Zhou, Lu, Xie: "Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein." in: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 14, pp. 2166-74, (2011) (PubMed).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Molecular Weight: 68255

Gene ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase