Hexokinase 2 antibody (AA 401-431)

Catalog No. ABIN392756

The Rabbit Polyclonal anti-Hexokinase 2 antibody is suitable to detect Hexokinase 2 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Hexokinase 2 antibody (AA 401-431) (ABIN392756)

Target: Hexokinase 2 (HK2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB03920

Product Details anti-Hexokinase 2 Antibody

Binding Specificity: AA 401-431

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-431 amino acids from the Central region of human HK2 (Hexokinase II).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HK2 (Hexokinase II)

Background: In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.

Molecular Weight: 102380

Gene ID: 3099

NCBI Accession: NP_000180

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect