Influenza Polymerase Acidic antibody (AA 250-300)

Catalog No. ABIN398948

Product Details anti-Influenza Polymerase Acidic Antibody

Target: Influenza Polymerase Acidic (PA)

Binding Specificity: AA 250-300

Reactivity: Influenza A Virus H1N1

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Influenza Polymerase Acidic antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: H1N1 PA Antibody detects influenza A (H1N1) PA protein.

Purification: Immunoaffinity chromatography.

Immunogen: KLH-coupled synthetic peptide from AA 250-300 of Influenza A virus PA protein (Genbank: CY040004).

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mL.Other Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Influenza Polymerase Acidic antibody (ABIN398948)

Pena, Vincent, Loving, Henningson, Lager, Li, Perez: "Strain-dependent effects of PB1-F2 of triple-reassortant H3N2 influenza viruses in swine." in: The Journal of general virology, Vol. 93, Issue Pt 10, pp. 2204-14, (2012) (PubMed).

Bradel-Tretheway, Mattiacio, Krasnoselsky, Stevenson, Purdy, Dewhurst, Katze: "Comprehensive proteomic analysis of influenza virus polymerase complex reveals a novel association with mitochondrial proteins and RNA polymerase accessory factors." in: Journal of virology, Vol. 85, Issue 17, pp. 8569-81, (2011) (PubMed).

Target Details for Influenza Polymerase Acidic

Target: Influenza Polymerase Acidic (PA)

Alternative Name: H1N1 PA (PA Products)

Synonyms: polymerase antibody, polymerase PA antibody, PA antibody

Target Type: Influenza Protein

Background: Influenza A (H1N1) virus, a subtype of influenzavirus A, is the most common cause of influenza (flu) in humans. A strain of swine-origin H1N1 was responsible for the 2009 flu pandemic. The Influenza A (H1N1) RNA polymerase consists of one part each of three subunits, PB1, PB2, and PA. PA is a subunit of RNA-dependent RNA polymerase that is essential for cRNA-dependent vRNA synthesis.
H1N1 PA Antibody is developed in rabbit using a KLH-coupled synthetic peptide from residues 250-300 of Influenza A virus PA protein (Genbank: CY040004).