CD22 antibody

Catalog No. ABIN400636

This Mouse Monoclonal antibody specifically detects CD22 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD22 antibody (ABIN400636)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: IS7

Product Details anti-CD22 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM, Sialic acid-binding Ig-like lectin 2,B-lymphocyte cell adhesion molecule

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Reh human cell line

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Positive control: lysed whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 μm filter sterilized.

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2

UniProt: P20273