JAG1 antibody (AA 110-125)

Catalog No. ABIN401024

The Rabbit Polyclonal anti-JAG1 antibody is suitable to detect JAG1 in samples from Human, Mouse, Rat and Chimpanzee. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for JAG1 antibody (AA 110-125) (ABIN401024)

Target: JAG1 (Jagged 1 (JAG1))

Reactivity: Human, Mouse, Rat, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-JAG1 Antibody

Binding Specificity: AA 110-125

Specificity: This protein A purified antibody is directed against Human Jagged-1 protein. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in Mouse liver whole cell lysates, and a 75 kDa band in Human brain and kidney lysates. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from Human, Chimpanzee, Rat and Mouse based on 100 % homology with the immunizing sequence. Partial reactivity is expected against dog (81 %) and Xenopus laevis (85 %) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Rat.
Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography on Protein A.

Immunogen: Synthetic peptide corresponding to amino acids 110-125 of Human Jagged-1 protein.

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence microscopy andWestern blot. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/500-1/2,000. Immunofluorescence: 1/200-1/1,000. Immunohistochemistry on Paraffin Sections: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: CD339 / JAG1

Background: Jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the Jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.Synonyms: JAGL1, Jagged-1, hJ1

Gene ID: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Notch Signaling, Stem Cell Maintenance