RNF25 antibody (C-Term)

Catalog No. ABIN401373

The Rabbit Polyclonal anti-RNF25 antibody (ABIN401373) specifically detects RNF25 in WB, IP and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for RNF25 antibody (C-Term) (ABIN401373)

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF25 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-RNF25 Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against RNF25 protein.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for RNF25

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Alternative Name: RNF25

Background: RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kappaB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25

Gene ID: 64320

NCBI Accession: NP_071898

UniProt: Q96BH1