Peptide ELISA: 1/8000. Western blot: Approx 160 kDa band observed in Human Breast Cancer lysates (calculatedMW of 160 kDa according to NP_002947.1. Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Thyroid Gland shows structuredcytoplasm staining in active epithelial cells. Recommended concentration: 2 - 4 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Target
CLIP1
(CAP-GLY Domain Containing Linker Protein 1 (CLIP1))
CLIP170 associates with the growing ends of microtubules, a type of behaviour known as 'plus end tracking' that is exhibited by proteins called +TIPS. It is known to bind to CLASPS and IQGAP1, and is thought to play a role as a positive regulator of microtubule dynamics. CLIP170 is also involved in targeting of the dynactin complex to the ends of growing microtubules, and may be important in mitosis.Synonyms: CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, RSN, Reed-Sternberg intermediate filament-associated protein