CHEK1 antibody (pSer280)

Catalog No. ABIN401536

This Rabbit Polyclonal antibody specifically detects CHEK1 in WB. It exhibits reactivity toward Human.

Quick Overview for CHEK1 antibody (pSer280) (ABIN401536)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHEK1 Antibody

Binding Specificity: pSer280

Specificity: This antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 280.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280 (V-T-Sp-G-G).

Application Details

Application Notes: Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Gene ID: 1111

NCBI Accession: NP_001107593

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair