His Tag antibody (Biotin)

Catalog No. ABIN401733

The Biotin-conjugated Rabbit Polyclonal anti-His Tag antibody (ABIN401733) specifically detects His Tag in WB, IHC (fro) and EIA.

Quick Overview for His Tag antibody (Biotin) (ABIN401733)

Target: His Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-His Tag Antibody (Biotin)

Sequence: HHHHHH

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Affinity Chromatography

Immunogen: 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide

Application Details

Application Notes: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X Hisepitope. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for Immunoprecipitation andImmunocytochemistry. Recommended Dilutions:

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent). For extended storage, mix with Glycerol to 50 % final

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for His Tag

Target: His Tag

Alternative Name: 6xHistidine Epitope Tag

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.