BrdU antibody

Catalog No. ABIN4219249

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Please inquire

Specificity: Bromodeoxyuridine

Purification: Protein A affinity column

Immunogen: Bromodeoxyuridine

Clone: BU5-1

Isotype: IgG2a

Application Details

Application Notes: Detection of bromodeoxyuridine incorporation in Tasse and cell culture (1:10)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: reconstitue with 1 mL deionized water

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 2-8°C, extended storage at -20°C, avoid repeated freeze-thaw cycles

References for anti-BrdU antibody (ABIN4219249)

Jirikowski, Ramalho-Ortigao, Kesse, Bloom: "In situ hybridization of semithin Epon sections with BrdU labelled oligonucleotide probes." in: Histochemistry, Vol. 94, Issue 2, pp. 187-90, (1990) (PubMed).

Hoshino, Nagashima, Murovic, Levin, Levin, Rupp: "Cell kinetic studies of in situ human brain tumors with bromodeoxyuridine." in: Cytometry, Vol. 6, Issue 6, pp. 627-32, (1986) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: Bromodeoxyuridine (BrdU Products)

Target Type: Chemical

Background: The antibody is specific for bromodesoxyuridine and does not cross-react with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell ad tissue culture. 3H-thymidine labelling techniques can be avoided.