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p53 antibody (AA 20-31)

This anti-p53 antibody is a Mouse Monoclonal antibody detecting p53 in WB, ELISA, IP, IHC (fp) and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 9+ publications.
Catalog No. ABIN4219254

Quick Overview for p53 antibody (AA 20-31) (ABIN4219254)

Target

See all p53 (TP53) Antibodies
p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity

  • 957
  • 277
  • 229
  • 140
  • 74
  • 72
  • 57
  • 39
  • 10
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  • 2
  • 1
  • 1
Human

Host

  • 671
  • 312
  • 5
  • 2
  • 2
Mouse

Clonality

  • 576
  • 415
  • 1
Monoclonal

Conjugate

  • 519
  • 51
  • 31
  • 20
  • 19
  • 17
  • 17
  • 17
  • 17
  • 17
  • 17
  • 17
  • 14
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  • 14
  • 14
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 10
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
This p53 antibody is un-conjugated

Application

  • 726
  • 322
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  • 80
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  • 32
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  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

Bp53-11
  • Binding Specificity

    • 39
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    • 31
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    • 26
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    • 22
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    AA 20-31

    Specificity

    P53

    Cross-Reactivity

    Human

    Purification

    Protein A affinity column

    Immunogen

    Recombinant human p53 (transcription domain within the NH2 terminus)

    Isotype

    IgG2a
  • Application Notes

    IHC: 1:500, when using the APAAP method

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    reconstitue with 1 mL deionized water

    Concentration

    0.025 mg/mL

    Buffer

    PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C/-20 °C

    Storage Comment

    2-8°C, extended storage at -20°C, avoid repeated freeze-thaw cycles
  • Zarka, Han, Edelson, Rosenblum: "Expression of cadherins, p53, and BCL2 in small cell carcinomas of the cervix: potential tumor suppressor role for N-cadherin." in: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, Vol. 13, Issue 2, pp. 240-3, (2003) (PubMed).

    Ohtaké, Cheng, Ida, Suzuki, Ohshiro, Zhang, Saku: "Precancerous foci in pleomorphic adenoma of the salivary gland: recognition of focal carcinoma and atypical tumor cells by P53 immunohistochemistry." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 31, Issue 10, pp. 590-7, (2002) (PubMed).

    Homann, Nees, Conradt, Dietz, Weidauer, Maier, Bosch: "Overexpression of p53 in tumor-distant epithelia of head and neck cancer patients is associated with an increased incidence of second primary carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 2, pp. 290-6, (2001) (PubMed).

    Andl, Kahn, Pfuhl, Nicola, Erber, Conradt, Klein, Helbig, Dietz, Weidauer, Bosch: "Etiological involvement of oncogenic human papillomavirus in tonsillar squamous cell carcinomas lacking retinoblastoma cell cycle control." in: Cancer research, Vol. 58, Issue 1, pp. 5-13, (1998) (PubMed).

    Petersen, Song, Hügle-Dörr, Oldenburg, Bautz: "Mapping of linear epitopes recognized by monoclonal antibodies with gene-fragment phage display libraries." in: Molecular & general genetics : MGG, Vol. 249, Issue 4, pp. 425-31, (1996) (PubMed).

    Stephen, Helminen, Lane: "Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions." in: Journal of molecular biology, Vol. 248, Issue 1, pp. 58-78, (1995) (PubMed).

    Fisher, Gillett, Vojtĕsek, Barnes, Millis: "Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation." in: British journal of cancer, Vol. 69, Issue 1, pp. 26-31, (1994) (PubMed).

    Bártek, Bártková, Lukás, Stasková, Vojt?sek, Lane: "Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies." in: The Journal of pathology, Vol. 169, Issue 1, pp. 27-34, (1993) (PubMed).

    Bártek, Bártková, Vojtĕsek, Stasková, Rejthar, Kovarík, Lane: "Patterns of expression of the p53 tumour suppressor in human breast tissues and tumours in situ and in vitro." in: International journal of cancer, Vol. 46, Issue 5, pp. 839-44, (1990) (PubMed).

  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Alternative Name

    P53

    Background

    Excellent marker for wild-type and mutant forms of human p53 antigen. The antibody stains positively approx. 60 % of investigated carcinoma of lung, breast, colon, stomach, esophagus, pancreas, urinary bladder and testis, head and neck tumors, T-cell Leuk

    Gene ID

    7157

    UniProt

    P04637

    Pathways

    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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