CDK7 antibody (C-Term)

Catalog No. ABIN452138

Product Details anti-CDK7 Antibody

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Specificity: Mo-1.1 recognizes the key cell cycle regulator cdk7, which is essential to activate the cdk2 kinase in G1 phase and the cdc2 kinase in G2/M phase.

Characteristics: Synonyms: CAK1, P39 Mo15, STK1, CDK-activating kinase, Cell division protein kinase 7, TFIIH basaltranscription factor complex kinase subunit, 39 kDa protein kinase

Purification: Protein A affinity chromatography

Immunogen: Recombinant C-terminal 221 aa fragment of human wild-type cdk7/CAK (cyclin activatingkinase)

Clone: MO-1-1

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry of frozen sections: 1/10 - 1/20, 1h at RT. Immunofluorescence. Immunoprecipitation of active kinase complex. Cell sorting (FACS analysis). Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water (final solution contains 0.09 % NaN3, 0.5 % BSA in PBS buffer, pH 7.4).

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CDK7

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Alternative Name: CDK7 (CDK7 Products)

Background: Cdk7 is a member of the cyclin dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. Cdk7 forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.Synonyms: 39 kDa protein kinase, CAK1, CDK-activating kinase, Cell division protein kinase 7, P39 Mo15, STK1, TFIIH basal transcription factor complex kinase subunit

Gene ID: 1022

UniProt: P50613

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, M Phase