Prostate Specific Antigen antibody (free)

Catalog No. ABIN452575

This Mouse Monoclonal antibody specifically detects Prostate Specific Antigen in EIA and ELISA (Capture). It exhibits reactivity toward Human.

Quick Overview for Prostate Specific Antigen antibody (free) (ABIN452575)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: 108

Product Details anti-Prostate Specific Antigen Antibody

Binding Specificity: free

Specificity: This monoclonal antibody reacts with Human free PSA, but does not react with complexed PSA.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Prostate-specific antigen, Semenogelase, Gamma-seminoprotein, P-30 antigen

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified Human Free PSA.

Isotype: IgG2a

Application Details

Application Notes: ELISA: This monoclonal PSA antibody can be used as Capture antibody in a SandwichELISA for Human free PSA detection in combination with a HRP-conjugated Tracer antibody. Suggested Capture Coating Dose: 0.5 μg/well. Substrate: TMB. If the above suggestedconditions are followed approximately 22 pg/mL of Free PSA can be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distilled water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: Kallikrein-3 / PSA / KLK3

Background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.Synonyms: Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase

Gene ID: 354

UniProt: P07288

Pathways: Complement System