TYMS antibody (N-Term)

Catalog No. ABIN452662

This Mouse Monoclonal antibody specifically detects TYMS in WB, IF and EIA. It exhibits reactivity toward Human.

Quick Overview for TYMS antibody (N-Term) (ABIN452662)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: AT1S5

Product Details anti-TYMS Antibody

Binding Specificity: AA 1-313, N-Term

Specificity: The antibody recognizes human TYMS. Other species not tested.

Characteristics: Synonyms: TYMS, TSase, OK/SW-cl.29

Purification: Protein-G affinity chromatography

Immunogen: Recombinant TYMS (1-313 aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (1: 500 - 1: 1,000). Immunofluorescence (1: 500 - 1: 1,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase (TS)

Background: The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS). TYMS is an intracellular enzyme that provides the sole de novo source of thymidylate in activity highest proliferating cells. Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head and neck. TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers.Synonyms: OK/SW-cl.29, TSase, TYMS

Gene ID: 54583

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases