Actin antibody

Catalog No. ABIN457424

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC (p), ICC and IHC (fro). It is suitable to detect Actin in samples from Human, Mouse, Rat, Rabbit, Chicken, Dog, Non-Human Primate and Cat. There are 9+ publications available.

Quick Overview for Actin antibody (ABIN457424)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Dog, Non-Human Primate, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HHF35

Product Details anti-Actin Antibody

Purpose: Anti-Actin (muscle) Purified

Specificity: The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: SDS extracted protein fraction of human myocardium

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL, positive control: murine femoral muscle, murine heart, negative control: HUVEC line, reducing conditions.
Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Actin antibody (ABIN457424)

Matsuo, Susumu, Tsutsumi, Azuma, Obata, Hayashi: "Glomus tumor of the omentum: a case report." in: Journal of surgical oncology, Vol. 96, Issue 7, pp. 633-6, (2007) (PubMed).

Matsuyama, Hisaoka, Hashimoto: "Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma." in: Human pathology, Vol. 38, Issue 4, pp. 645-51, (2007) (PubMed).

Ijzer, Roskams, Molenbeek, Ultee, Penning, Rothuizen, van den Ingh: "Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver." in: Comparative hepatology, Vol. 5, pp. 7, (2006) (PubMed).

Handharyani, Ochiai, Iwata, Umemura: "Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 5, pp. 547-52, (2001) (PubMed).

de Sousa, Schwarzschild, de Araújo, de Araújo: "Basal cell adenocarcinoma of the palate with squamous metaplasia." in: Journal of clinical pathology, Vol. 53, Issue 2, pp. 153-6, (2000) (PubMed).

Miettinen: "Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors." in: The American journal of pathology, Vol. 130, Issue 1, pp. 205-15, (1988) (PubMed).

Schmidt, Cone, Haas, Gown: "Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins." in: The American journal of pathology, Vol. 131, Issue 1, pp. 19-28, (1988) (PubMed).

Tsukada, McNutt, Ross, Gown: "HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues." in: The American journal of pathology, Vol. 127, Issue 2, pp. 389-402, (1987) (PubMed).

Tsukada, Tippens, Gordon, Ross, Gown: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." in: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, (1987) (PubMed).

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Actin (muscle)

Background: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.,Muscle specific actin

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development