Albumin antibody (Biotin)

Catalog No. ABIN457583

The Rabbit Polyclonal anti-Albumin antibody is suitable to detect Albumin in samples from Cow. It has been validated for WB, ELISA, ICC, IHC (p) and DB.

Quick Overview for Albumin antibody (Biotin) (ABIN457583)

Target: Albumin (ALB)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Product Details anti-Albumin Antibody (Biotin)

Purpose: Rabbit anti Bovine albumin, conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine albumin.

Cross-Reactivity: Dog, Goat, Horse, Human, Monkey, Pig, Sheep

No Cross-Reactivity: Chicken, Mouse, Rabbit

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Chicken - Dog + Goat ++ Guinea Pig ± Horse + Human + Monkey + Mouse - Rabbit - Rat ± Sheep ++ Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Characteristics: The defined antibody specificity is directed to albumin as tested against bovine sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin. In immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, in non-isotopic assay methodology (e.g. ELISA) to measure albumin in bovine serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Pathways: Lipid Metabolism