beta-2 Microglobulin antibody

Catalog No. ABIN458094

The Rabbit Polyclonal anti-beta-2 Microglobulin antibody (ABIN458094) specifically detects beta-2 Microglobulin in IEP, ODD and RID. The antibody is reactive with Human samples.

Quick Overview for beta-2 Microglobulin antibody (ABIN458094)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD), Radial Immunodiffusion (RID)

Product Details anti-beta-2 Microglobulin Antibody

Purpose: Rabbit anti Human beta-2 microglobulin

Specificity: Precipitating polyclonal Rabbit antiSerum to Human beta-2 microglobulin.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail

Characteristics: The defined antibody reactivity is restricted to beta-2 microglobulin as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained with foetal serum, colostrum and selected pathological plasma and urine containing high levels of beta-2 microglobulin in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of beta-2 microglobulin and to determine its concentration. The presence of non-precipitating antibodies has not been assayed. It does not exclude the use of the antiserum in non-precipitating techniques if proper controls are included.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Beta-2 microglobulin is a member of the Immunoglobulin superfamily. The amino acid sequence resembles that of immunoglobulin constant domains. It is isolated from urine of patients with impaired readsorption in the kidney tubules. High levels of beta-2 microglobulin are as present in cord blood serum and foetal serum. The host animal is immunised with highly purified beta-2 microglobulin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL serum, plasma or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta-2 Microglobulin

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process