Secretory Component antibody (Soluble)

Catalog No. ABIN458147

Product Details anti-Secretory Component Antibody

Target: Secretory Component

Binding Specificity: Soluble

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Secretory Component antibody is un-conjugated

Application: Immunoelectrophoresis (IEP)

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homo-logous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail. It cross-reacts with free secretory component in secretions of chimpanzee and other non-human primates including Rhesus monkeys.

Characteristics: Precipitating polyclonal rabbit antiserum to free human secretory component

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized sIgA as required to eliminate antibody activity to surface determinants. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from immunoglobulin deficient milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion to identify the presence of free secretory component in human serum or other body fluids and to determine its concentration.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against immunoglobulin deficient milk in agar-block immuno-diffusion

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for Secretory Component

Target: Secretory Component

Abstract: Secretory Component Products

Synonyms: RNPIGR2 antibody, pIgA-R antibody, polymeric immunoglobulin receptor antibody, PIGR antibody, Pigr antibody

Background: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electro-endosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with sIgA, or with any other secretory or plasma protein