SERPINA3
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))
Reactivity
Human
Host
Sheep
Clonality
Polyclonal
Conjugate
This SERPINA3 antibody is un-conjugated
Application
Immunoelectrophoresis (IEP)
Specificity
The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
Characteristics
Precipitating polyclonal sheep antiserum to human alpha-1 antichymotrypsin
Purification
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogen
Alpha-1 antichymotrypsin is a protease inhibitor with a molecular weight of 68.000. Its con-centration in fetal human serum is low at birth, but the concentration increases rapidly to normal plasma levels of about is 4.5 µ g/ml. Alpha-1 antichymotrypsin is regulated like a an acute phase reactant. It has been isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
SERPINA3
Reactivity: Human, Mouse, Rat
IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of alpha-1 antichymotrypsin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
Total protein and IgG concentration are comparable to those of pooled normal sheep serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar-block immunodiffusion titration. No foreign proteins added.
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
Target
SERPINA3
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))
SERPINA3-1 antibody, Pi2 antibody, AACT antibody, ACT antibody, GIG25 antibody, A1AT antibody, PI antibody, alpha-1-PI antibody, AAT antibody, Pi antibody, Spi1 antibody, SERPINA3-5 antibody, serpina3 antibody, serpin A3-5 antibody, serpin family A member 3 antibody, alpha-1-antiproteinase F precursor antibody, serpin family A member 1 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 antibody, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog antibody, LOC396684 antibody, SERPINA3 antibody, LOC100008973 antibody, SERPINA1 antibody, Serpina1 antibody, SPIA5 antibody, serpina3.L antibody
Background
The reactivity of the antiserum is restricted to alpha-1 antichymotrypsin. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen