Lactoferrin antibody (Biotin)

Catalog No. ABIN458279

Product Details anti-Lactoferrin Antibody (Biotin)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antiserum does not cross-react with any other monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail , however a strong cross-reaction with lactoferrin in human milk has been observed.

Characteristics: Biotin-conjugated IgG fraction of polyclonal goat antiserum to monkey lactoferrin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized fractions of monkey serum and lactoferrin-depleted monkey milk as required, to eliminate antibodies reacting with other monkey serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In immunocytochemical and immunohistochemical techniques for the detection of monkey lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:7,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.2. No foreign proteins added.

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin (LTF Products)

Synonyms: LTF antibody, GIG12 antibody, HLF2 antibody, LF antibody, Lf antibody, Csp82 antibody, MMS10R antibody, Ms10r antibody, PLF antibody, TFEW antibody, conalbumin antibody, lactotransferrin antibody, transferrin antibody, transferrin (ovotransferrin) antibody, LTF antibody, CpipJ_CPIJ000114 antibody, TF antibody, Ltf antibody

Background: Tested in immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma

Pathways: Transition Metal Ion Homeostasis