IgA Secretory Component (Free & Bound) antibody

Catalog No. ABIN458282

There is 1 publication for this product available. The Goat Polyclonal anti-IgA Secretory Component antibody is suitable to detect IgA Secretory Component in samples from Monkey. It has been validated for ELISA, IEP and RID.

Quick Overview for IgA Secretory Component (Free & Bound) antibody (ABIN458282)

Target: IgA Secretory Component

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Product Details

Binding Specificity: Free & Bound

Purpose: Goat anti Monkey secretory component (free and bound)

Specificity: Precipitating polyclonal goat antiserum to monkey secretory component.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The antiserum may also react with other species as has been observed for Chimpanzee.

Characteristics: Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

References

Thomas, Tuero, Demberg, Vargas-Inchaustegui, Musich, Xiao, Venzon, LaBranche, Montefiori, DiPasquale, Reed, DeVico, Fouts, Lewis, Gallo, Robert-Guroff: "HIV-1 CD4-induced (CD4i) gp120 epitope vaccines promote B and T-cell responses that contribute to reduced viral loads in rhesus macaques." in: Virology, Vol. 471-473, pp. 81-92, (2015) (PubMed).

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)