Albumin antibody (HRP)

Catalog No. ABIN458444

The Rabbit Polyclonal anti-Albumin antibody is suitable to detect Albumin in samples from Mouse. It has been validated for WB, ELISA, ICC, IHC (p) and DB.

Quick Overview for Albumin antibody (HRP) (ABIN458444)

Target: Albumin (ALB)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Product Details anti-Albumin Antibody (HRP)

Purpose: Rabbit anti Mouse albumin, conjugated with Horseradish peroxidase

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse albumin

Cross-Reactivity: Hamster, Horse, Human, Monkey, Pig, Rat

No Cross-Reactivity: Chicken, Dog, Goat, Sheep

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: cow - Goat - Horse + Rat ++ Chicken - Guinea Pig ++ Human + Sheep - Dog - Hamster + Monkey + Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Characteristics: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal mouse serum and purified mouse albumin. One characteristic precipitin line is obtained against pooled normal mouse serum using different antigen/antibody concentration ratio's. Precipitin lines against normal mouse serum and purified mouse albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in mouse serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from mouse serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Pathways: Lipid Metabolism