Fibrinogen antibody

Catalog No. ABIN458504

The Goat Polyclonal anti-Fibrinogen antibody has been validated for WB, ELISA, IHC, ICC and IF. It is suitable to detect Fibrinogen in samples from Mouse. There are 2+ publications available.

Quick Overview for Fibrinogen antibody (ABIN458504)

Target: Fibrinogen

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Fibrinogen antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Fibrinogen Antibody

Specificity: The antiserum does not cross-react with any other component of mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Purified IgG fraction of polyclonal goat antiserum to mouse fibrinogen

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Physicochemical characteristic IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

References for anti-Fibrinogen antibody (ABIN458504)

Zang, Gustafsson, Jamalpour, Hong, Genové, Welsh: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." in: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).

Tugues, Roche, Noguer, Orlova, Bhoi, Padhan, Akerud, Honjo, Selvaraju, Mazzone, Tolmachev, Claesson-Welsh: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." in: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).

Target Details for Fibrinogen

Target: Fibrinogen

Background: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products