Albumin antibody (HRP)

Catalog No. ABIN458654

The Goat Polyclonal anti-Albumin antibody is suitable to detect Albumin in samples from Rat. It has been validated for WB, ELISA, ICC, IHC (p) and DB.

Quick Overview for Albumin antibody (HRP) (ABIN458654)

Target: Albumin (ALB)

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Product Details anti-Albumin Antibody (HRP)

Purpose: Goat anti Rat albumin, conjugated with Horseradish peroxidase

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rat albumin.

Cross-Reactivity: Dog, Horse, Human, Monkey, Mouse, Pig

No Cross-Reactivity: Chicken, Cow, Goat, Sheep

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Mouse ++ Sheep - Dog + Horse + Monkey + Swine + A negative reaction in double radial immunodiffusion does not exclude a weak positive reaction in more sensitive techniques.

Characteristics: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in rat serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Purified albumin isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

UniProt: P02770

Pathways: Lipid Metabolism