Albumin antibody (FITC)

Catalog No. ABIN458723

The Rabbit Polyclonal anti-Albumin antibody is suitable to detect Albumin in samples from Rat. It has been validated for ELISA, ICC, IHC (fro) and IFA.

Quick Overview for Albumin antibody (FITC) (ABIN458723)

Target: Albumin (ALB)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to FITC

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Product Details anti-Albumin Antibody (FITC)

Purpose: Rabbit anti Rat albumin, conjugated with FITC

Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Rat albumin.

Cross-Reactivity: Horse, Mouse, Pig

No Cross-Reactivity: Chicken, Cow, Dog, Goat, Rabbit, Sheep

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross- reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Chicken - Dog - Goat - Guinea Pig ++ Horse + Human ± Monkey ± Mouse ++ Rabbit - Sheep - Swine ±

Characteristics: The defined antibody specificity is directed to albumin as tested against rat sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. As reagent for the direct detection of albumin in rat cells, tissues and body fluids in immunofluorescence techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Highly purified albumin isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Pathways: Lipid Metabolism