Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Enolase antibody (Biotin)

Reactivity: Rabbit ELISA, WB, IF Host: Sheep Polyclonal Biotin
Catalog No. ABIN459122
  • Target See all Enolase Antibodies
    Enolase
    Reactivity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 3
    • 2
    • 1
    Sheep
    Clonality
    • 3
    • 3
    Polyclonal
    Conjugate
    • 6
    This Enolase antibody is conjugated to Biotin
    Application
    • 5
    • 4
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    Biotin-conjugated IgG fraction of polyclonal sheep antiserum to enolase from rabbit muscle
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Enolase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
    Top Product
    Discover our top product Enolase Primary Antibody
  • Application Notes
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.7. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    Enolase
    Abstract
    Enolase Products
    Synonyms
    BEST:LD15491 antibody, CG17654 antibody, CT32526 antibody, Dmel\\CG17654 antibody, ENOA antibody, Eno2 antibody, T18 antibody, anon-WO0153538.76 antibody, eno antibody, ECK2773 antibody, JW2750 antibody, T22I11.6 antibody, T22I11_6 antibody, Enolase antibody, enolase antibody, phosphopyruvate hydratase antibody, O-methyltransferase family protein antibody, Eno antibody, eno antibody, Dvir\Eno antibody, eno-4 antibody, MMAH_RS09975 antibody, IGMT3 antibody
    Background
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
You are here:
Support