TPI1 antibody (Biotin)

Catalog No. ABIN459134

Product Details anti-TPI1 Antibody (Biotin)

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This TPI1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to triosephosphate isomerase from rabbit muscle

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Triosephosphate isomerase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 7.4. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: Triosephosphate Isomerase (TPI1 Products)

Synonyms: TIM antibody, TPI antibody, TIM1 antibody, hTIM antibody, tpi antibody, tpi1 antibody, TPI1 antibody, AI255506 antibody, Tpi antibody, Tpi-1 antibody, GB17473 antibody, Tim antibody, CG2171 antibody, Dmel\\CG2171 antibody, ND14 antibody, TPIS antibody, sgk antibody, wstd antibody, DrTPI-A antibody, DrTPI-B antibody, TIM-B antibody, cb179 antibody, wu:fb51c02 antibody, triosephosphate isomerase 1 antibody, timeless circadian regulator antibody, triosephosphate isomerase 1 S homeolog antibody, triosephosphate isomerase antibody, triose-phosphate isomerase antibody, Triose-phosphate isomerase antibody, triose-phosphate isomerase, putative antibody, timeless circadian clock antibody, Triose phosphate isomerase antibody, Triosephosphate isomerase antibody, triosephosphate isomerase 1a antibody, triosephosphate isomerase 1b antibody, TPI1 antibody, TIMELESS antibody, tpi1 antibody, Tpi1 antibody, tpi1.S antibody, Tpi antibody, tpiA antibody, CNE05000 antibody, Fnod_0738 antibody, Tlet_0996 antibody, SARE_RS16215 antibody, PKH_126640 antibody, Timeless antibody, tpi-1 antibody, tpi1a antibody, tpi1b antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Cell RedoxHomeostasis