CAMK2B antibody

Catalog No. ABIN462429

The Rabbit Polyclonal anti-CAMK2B antibody (ABIN462429) specifically detects CAMK2B in WB, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Cow, Horse, Rabbit, Monkey, Zebrafish (Danio rerio), Dog, Xenopus laevis, Chicken and Pig samples.

Quick Overview for CAMK2B antibody (ABIN462429)

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivity: Human, Rat, Mouse, Cow, Horse, Rabbit, Monkey, Zebrafish (Danio rerio), Dog, Xenopus laevis, Chicken, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CAMK2B Antibody

Specificity: Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%) Orangutan (93%).

Purification: Immunoaffinity purified

Immunogen: A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%), Orangutan (93%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Alternative Name: CAMK2 / CAMKII

Background: Name/Gene ID: CAMK2

Synonyms: CAMK2

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity