NCOA3 antibody (AA 188-198)

Catalog No. ABIN462508

This Goat Polyclonal antibody specifically detects NCOA3 in IHC and IHC (p). It exhibits reactivity toward Human, Cow, Dog, Pig, Monkey, Bat and Hamster.

Quick Overview for NCOA3 antibody (AA 188-198) (ABIN462508)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Human, Cow, Dog, Pig, Monkey, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This NCOA3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCOA3 Antibody

Binding Specificity: AA 188-198

Brand: IHC-plus™

Specificity: Human NCOA3. This antibody is expected to recognise both reported isoforms (NP_006525.2, NP_858045.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig (100%) Mouse, Rat, Horse, Rabbit (91%) Opossum, Turkey, Platypus, Lizard (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TNETQRQKSHT from an internal region of human NCOA3 (NP_006525.2, NP_858045.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig (100%), Mouse, Rat, Horse, Rabbit (91%), Opossum, Turkey, Platypus, Lizard (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: NCOA3 / SRC-3 / AIB1

Background: Name/Gene ID: NCOA3

Synonyms: NCOA3, ACTR, AIB1, BHLHe42, CAGH16, CBP-interacting protein, CTG26, AIB-1, P/CIP, TNRC14, TRAM-1, Nuclear receptor coactivator 3, PCIP, RAC-3, SRC3, TNRC16, KAT13B, NCoA-3, SRC-3

Gene ID: 8202

NCBI Accession: NP_006525, NP_858045

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha