IL12 antibody (Biotin)

Catalog No. ABIN465093

The Goat Polyclonal anti-IL12 antibody has been validated for ELISA, WB and Neut. It is suitable to detect IL12 in samples from Mouse.

Quick Overview for IL12 antibody (Biotin) (ABIN465093)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-IL12 Antibody (Biotin)

Specificity: Mouse IL-12

Purification: Immunoaffinity purified

Immunogen: CHO-cell derived recombinant Murine IL-12.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mIL-12 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-12. To detect mIL-12 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-12 (LS-C104421) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-12. Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12 / IL-12

Background: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome