CCL2 antibody
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- Target See all CCL2 Antibodies
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CCL2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human MCP-1/MCAF
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human MCP-1/MCAF.
Type of Immunogen: Cell extract - Isotype
- IgG1
- Top Product
- Discover our top product CCL2 Primary Antibody
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- Application Notes
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Approved: ELISA (2 - 4 μg/mL), WB (0.2 - 0.4 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human MCP-1 when used with biotinylated antigen affinity purified anti-human MCP-1 (LS-C104657) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1-2 ng/lane, under reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Alternative Name
- CCL2 / MCP-1 (CCL2 Products)
- Synonyms
- GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody
- Background
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Name/Gene ID: CCL2
Subfamily: Intercrine beta
Family: Intercrine
Synonyms: CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF - Gene ID
- 6347
- UniProt
- P13500
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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