RELM alpha antibody

Catalog No. ABIN465191

The Rabbit Polyclonal anti-RELM alpha antibody is suitable to detect RELM alpha in samples from Mouse. It has been validated for WB and ELISA.

Quick Overview for RELM alpha antibody (ABIN465191)

Target: RELM alpha

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RELM alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RELM alpha Antibody

Specificity: Mouse RELMalpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine RELMalpha.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMalpha (LS-C104489) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMalpha. Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMalpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for RELM alpha

Target: RELM alpha

Alternative Name: Fizz1 / RELM Alpha

Background: Name/Gene ID: Retnla

Synonyms: Retnla, Fizz-1, Fizz1, HIMF, RELM Alpha, RELM-alpha, RELMa, RELMalpha, Resistin like alpha, Xcp2

Gene ID: 57262

UniProt: Q9EP95