AIMP1 / EMAP II antibody (Biotin)

Catalog No. ABIN465269

Product Details

Target: AIMP1 / EMAP II

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human EMAP-II

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human EMAP-II.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hEMAP-II by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEMAP-II. To detect hEMAP-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EMAP-II (LS-C104512) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEMAP-II. Western Blot: To detect hEMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEMAP-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile PBS with 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details

Target: AIMP1 / EMAP II

Background: Name/Gene ID: AIMP1

Synonyms: AIMP1, EMAP II, EMAPII, EMAP-2, HLD3, p43, EMAP2, SCYE1

Gene ID: 9255

UniProt: Q12904